Bacterial CRISPR and Fluorescent Yeast Combo kit
Excellent intro to synthetic biology.
Posted by Earl A. on 12th Dec 2018
Great kit, documentation and supplies. Did both experiments several times - all were successful. Very interesting introduction to synthetic biology especially if you supplement your study with additional materials. The book "Synthetic Biology" a Primer, Second Edition and the free Synthetic Biology 1 course at https://syntheticbiology1.com/courses/ are great for additional study.
This kit includes our best selling CRISPR kit and our yeast engineering kit at a $30 discount as opposed to when you buy them alone.
If the kit will be stored until Christmas please select the option to have the perishables shipped at a later date. We ship 2-3 day and last shipping day before Christmas will be Dec. 21.
Due to the overwhelming number of emails we will not respond to emails asking when your item will be shipped. Understand we are doing our best to get it to you.
These kits come with example experiments that teach you many molecular biology and gene engineering techniques.
Bacterial CRISPR Kit
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering? This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, tracrRNA, crRNA and Template DNA template for an example experiment.
Includes example experiment to make a genome mutation(K43T) to the rpsL gene changing the 43rd amino acid, a Lysine(K) to a Threonine(T) thereby allowing the bacteria to survive on Strep media which would normal prevent its growth.
Kit contains enough materials for around 5 experiments or more
- LB Agar
- LB Strep/Kan Agar
- Glass bottle for pouring plates
- Non-pathogenic E. coli bacteria
- Inoculation Loops/Plate Spreader
- 10-100uL variable volume adjustable pipette(1uL increments)
- Box of 96 Pipette Tips
- 14 Petri Plates
- Microcentrifuge tube rack
- Nitrile Gloves
- Microcentrifuge tubes
- 50mL Tube for measuring
- Bacterial transformation buffer 25mM CalCl2, 10% PEG 8000
- LB Media for transformation recovery
- Cas9 plasmid
- gRNA plasmid
- Template DNA
Some items in this kit need to be stored in a fridge and a freezer upon you receiving them.
Our goal with this kit is to begin to integrate synthetic biology and genetic design into people's everyday life. We see a future in which people are genetically designing the plants they use in their garden, eating yogurt that contains a custom bacterial strain they modified or even someday brewing using an engineered yeast strain.
Yeast is an integral part of our lives. It can used be used for brewing, baking, fermentation or as a research tool. Genetically Engineering yeast in your home seems like Science Fiction but is actually now reality. Using our kit you can make your yeast fluoresce and glow by inserting a gene from a jellyfish, the Green Flourescent Protein(GFP). This kit comes with everything you need to engineer a Mead Yeast we provide or your own favorite yeast that you provide. Most any yeast you can buy from a store you can engineer!
That is what makes this kit so powerful is that you can engineer laboratory yeast, yeast isolated from the environment, brewing yeast, baking yeast or even maybe yeast from your skin!
The kit uses a genetically designed extra chromosomal DNA(plasmid) that contains a form of the Green Fluorescent Protein. This protein makes it so that when you expose the engineered yeast to a "black light" or blue light and the yeast glow as seen in the picture.
Check out these articles written about this product by clicking on the logos below
This yeast and plasmid are being released through a Creative Commons Non-Commercial License we encourage you to share it. For commercial use we ask that you contact us for a license at firstname.lastname@example.org.
1 - YPD Agar containing G418
14 - Petri Plates
5 - Pairs of Nitrile Gloves
5 - Inoculation Loops
1 - Plate spreader
25~ - microcentrifuge tubes
6 - 1.5mL microfuge tubes containing YPD
1 mL Yeast transformation buffer 40% PEG 8000, 200mM LiAc, 0.1mg/mL Salmon Sperm DNA
1 - Preengineered strain of Yeast Containing GFP Plasmid
1 - UV Filter glasses and blue light