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DIY Bacterial Gene Engineering CRISPR Kit
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DIY Bacterial Gene Engineering CRISPR Kit

$199.00 $169.00
(26 reviews) Write a Review
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Description

If you are looking to use this kit to learn molecular biology and genetic engineering we instead recommend that you purchase our Learn Bioengineering Class which includes this kit as part of it. You can select payment plan on check out for the class to split the cost into 4 monthly payments of $80 each.

 

We Fulfill and Ship in 2-3 days in the US. Overnight is not required and neither are ice packs.

Due to the overwhelming number of emails we will not respond to emails asking when your item will be shipped. Understand we are doing our best to get it to you.

Comes with an example experiment that teaches you many molecular biology and gene engineering techniques.

Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering? This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, gRNA and Template DNA template for an example experiment.

Includes example experiment to make a genome mutation(K43T) to the rpsL gene changing the 43rd amino acid, a Lysine(K) to a Threonine(T) thereby allowing the bacteria to survive on Strep media which would normal prevent its growth.

Kit contains enough materials for around 5 experiments or more

  • LB Agar
  • LB Strep/Kan/Arabinose Agar
  • Glass bottle for pouring plates
  • Non-pathogenic E. coli DH5a bacteria
  • Inoculation Loops/Plate Spreader
  • 10-100uL variable volume adjustable pipette(1uL increments)
  • Box of 96 Pipette Tips
  • 14 Petri Plates
  • Microcentrifuge tube rack
  • Nitrile Gloves
  • Microcentrifuge tubes
  • 50mL Tube for measuring
  • Bacterial transformation buffer 25mM CalCl2, 20% PEG 8000
  • LB Media for transformation recovery
  • Cas9 plasmid
  • gRNA plasmid
  • Template DNA


**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**

 

Protocol For Experiment

 

 

You can find the plasmid DNA sequences

 

Cas9 Plasmid

 

gRNA Plasmid

 

 

Some items in this kit need to be stored in a fridge and a freezer upon you receiving them.

 

Reviews

  • 5
    best

    Posted by Unknown on 18th Aug 2021

    Vraiment top qualité jadore

  • 5
    Great Intro to Technique

    Posted by daniel t. on 30th Jul 2021

    We followed the instructions very carefully and got textbook results on the first go. We took the additional step of trying to grow the naive bug on strep agar (negative results). This kit is a great accompaniment to study of CRISPR. A concise summary of the processes needed to assemble this kit might give students a better feel for its elegance (how the template DNA is produced, for example--a lot of work is needed to put a student in a position to do this kit!). That is not a complaint. This is a really great and enlightening product.

  • 5
    Tech in the peoples hands

    Posted by 213 on 14th May 2021

    This is an amazing intro to science most would feel out of reach. The quality of materials is better than I had expected. Highly recommended

  • 4
    CRISPR in the 21st century

    Posted by Robert Pruitt on 30th Apr 2021

    The kit contains everything one needs to CRISPR some e. coli. If adequate pre-lab is done, it is quite understandable to most AP Bio students. Although it looks like a bacterial transformation that they have all done previously, they can understand what the Cas9 and the guide RNA is doing. I do have one recommendation - virtually all the kits I buy are for 8 stations/groups - this one is for 7. Drop some of the easily obtained or nice but unnessary things like gloves, 50 ml measuring device, and the microfuge tube rack, and give me enough of the nucleic acids, media, and bacteria to serve 8 stations.

  • 5
    amazing value

    Posted by Ian Cubit on 22nd Jan 2021

    The kit includes everything you need, with an easy to follow protocol for learning CRISPr. The price speaks for itself. 10/10

  • 5
    Very cool

    Posted by Amanda L on 28th Oct 2020

    Directions are a bit vague - yet I still succeed with zero experience! Highly recommend, very cool this is offered to the public.

  • 5
    Gift for my GF

    Posted by Antonio Caterino on 27th Oct 2020

    This is a gift for my GF, she study for become a biotechnologist and she is very happy when recive this kit. Tnx for all ODIN Sorry for my English but I come from Italy:)

  • 5
    A fantastic product with the UK shipping

    Posted by Aleksandra on 24th Aug 2020

    The Odin CRISPR/Cas9 bacterial kit is a creative and effective way for everyone who wants to learn biomedical engineering or biohacking. It has everything you need to perform a simple experiment and learn how to use basic laboratory equipment. Also, instructions are written in a very clear and engaging way and it has been shipped to the UK with no issues! Thanks the Odin!

  • 5
    Just what the doctor ordered

    Posted by Matthew Britton on 24th Jul 2020

    Great product

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