Edit Bacteria with CRISPR
$59.00 - $129.00
This kit comes with an example experiment that teaches you many molecular biology and gene engineering techniques.
However, if you are looking to use this kit to get a deeper understanding of bioengineering we recommend that you purchase our Learn Bioengineering Bundle which includes this kit as part of it.
We Fulfill and Ship in 2-3 days in the US. Overnight is not required and neither are ice packs.
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering? This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, gRNA and Template DNA template for an example experiment.
Includes example experiment to make a genome mutation(K43T) to the rpsL gene changing the 43rd amino acid, a Lysine(K) to a Threonine(T) thereby allowing the bacteria to survive on Strep media which would normal prevent its growth.
Kit contains everything you need for around 5 experiments or more
- LB Agar
- LB Strep/Kan/Arabinose Agar
- Glass bottle for pouring plates
- Non-pathogenic E. coli DH5a bacteria
- Inoculation Loops
- 10-100uL variable volume adjustable pipette(1uL increments)
- Box of 96 Pipette Tips
- 14 Petri Plates
- Microcentrifuge tube rack
- Nitrile Gloves
- Microcentrifuge tubes
- 50mL Tube for measuring
- Bacterial transformation buffer 25mM CalCl2, 20% PEG 8000
- LB Media for transformation recovery
- Cas9 plasmid
- gRNA plasmid
- Template DNA
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
Some items in this kit need to be stored in a fridge and a freezer upon you receiving them.
Take a look at our Experiment Protocol
Access plasmid maps.
Ordering the Refill/Classroom kit? Here's what it includes:
- Cas9 plasmid
- gRNA plasmid
- Template DNA
- Strep/Kan/Arabinose Agar Media
- LB Agar Media
- LB Media
- 20 microcentrifuge tubes
- 14 plates
- Non-pathogenic DH5a E. coli bacteria
- Inoculation loops
- Bacterial Transformation Buffer
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
*The REFILL KIT kit does not contain a pipette, pipette tips and a glass bottle for making media. These must be user supplied.
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Reviews
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excellent
I haven't receivend the kit yet, but I'm sure it will meet all my expectations. Thank you!
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Smooth and Straight-forward
AMAZING KIT! I got it super quickly. Inside there are Different elements to play with and explanation guide that teaches you how to work with CRISPR even-tough you have never used it before. I am having so much fun experimenting and exploring genome engineering!
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best
Vraiment top qualité jadore
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Great Intro to Technique
We followed the instructions very carefully and got textbook results on the first go. We took the additional step of trying to grow the naive bug on strep agar (negative results). This kit is a great accompaniment to study of CRISPR. A concise summary of the processes needed to assemble this kit might give students a better feel for its elegance (how the template DNA is produced, for example--a lot of work is needed to put a student in a position to do this kit!). That is not a complaint. This is a really great and enlightening product.
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Tech in the peoples hands
This is an amazing intro to science most would feel out of reach. The quality of materials is better than I had expected. Highly recommended
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CRISPR in the 21st century
The kit contains everything one needs to CRISPR some e. coli. If adequate pre-lab is done, it is quite understandable to most AP Bio students. Although it looks like a bacterial transformation that they have all done previously, they can understand what the Cas9 and the guide RNA is doing. I do have one recommendation - virtually all the kits I buy are for 8 stations/groups - this one is for 7. Drop some of the easily obtained or nice but unnessary things like gloves, 50 ml measuring device, and the microfuge tube rack, and give me enough of the nucleic acids, media, and bacteria to serve 8 stations.
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amazing value
The kit includes everything you need, with an easy to follow protocol for learning CRISPr. The price speaks for itself. 10/10
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Very cool
Directions are a bit vague - yet I still succeed with zero experience! Highly recommend, very cool this is offered to the public.
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Gift for my GF
This is a gift for my GF, she study for become a biotechnologist and she is very happy when recive this kit. Tnx for all ODIN Sorry for my English but I come from Italy:)