Glow-in-the-Dark Bacteria Engineering Kit
$39.99
Make bacteria glow green using a gene borrowed from jellyfish - then watch the results grow overnight on a petri dish.
Green Fluorescent Protein (GFP) won the Nobel Prize. Scientists use it to track genes, light up cancer cells, and visualize things invisible to the naked eye. In this kit, you'll put that exact gene into harmless E. coli - and see glowing colonies appear under blue light within 24 hours.
This is the experiment that hooks people on biology for life.
WHAT YOU'LL DO
- Rehydrate freeze-dried bacteria and GFP plasmid DNA
- Run a heat-shock bacterial transformation - the same technique used in real labs
- Grow your engineered bacteria overnight on selective agar plates
- Illuminate the results with the included blue light and UV filter
WHAT YOU'LL LEARN
- How fluorescent proteins work and why they matter in research
- The mechanics of bacterial transformation - how DNA gets into a cell
- Gene expression: why some bacteria glow and others don't
- How scientists use antibiotic selection to identify successful edits
PERFECT FOR
Total beginners. No lab experience needed. Works in a kitchen. Great for curious adults, teens, science fair projects, and classrooms.
NOTES
• All biological materials are freeze-dried and stable at room temperature for up to one month.
• Includes step-by-step transformation protocol and a guide to fluorescent proteins.
WHAT'S INCLUDED:
Biological Materials:
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Non-pathogenic E. coli (DH5α strain) bacteria, freeze dried
GFP plasmid containing jellyfish fluorescent gene (5 - 50uL of 20ng/uL)
Media & Reagents:
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LB agar media
LB selective agar (Kan 100 µg/ml)
Transformation buffer (25mM CaCl2, 10% PEG 8000)
LB recovery media
Lab Supplies:
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7 Petri plates
Transfer pipettes
Microcentrifuge tubes
Test tube for measuring 50 mL
UV Filter Sheet
Blue light
Agar preparation bottle
5 Sterile inoculation loops
10 nitrile gloves
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
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Reviews
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New and Improved
I love the new and improved jellyfish genetics kit! I ordered one last year to use in my 7th grade science classroom. The issues I had with the previous kit, such as scattered materials and convoluted instructions, seem to be fixed with the new iteration of the kit. I love that it comes with links to instructional videos and materials. My kids have a blast doing their genetic design projects each year!
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Success on 2nd attempt
Messed up what bacteria to grow at the start so re-did and had success. The folks at Odin rock, love the vids and weekly updates, I'll be back for more.
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Just do it.
This kit does require a little concentration and I found that things took a little longer than described, so...patience. Doing this kit force me to learn A LOT about how genes work and this is what I wanted. It's pretty simple but requires a number of steps. I'd recommend giving things a couple days to happen. It took a few days to get my bacteria to grow etc. But, hey, what's the hurry. Be careful who you about this. Some people have have very strong opinions about anything like this and they think you're some sort of evil scientist just for trying to learn something.
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Fun, but a little more detail would help
My 10 yr old son and I did this as a fun summer activity. The steps were clearly spelled out. However, I wish the instructions were a bit more detailed. He would have learned more if explanations about each "ingredient" were given, including details about the different types of agar and why they matter.
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Some delay but it worked
The manual says that it could take 24 to 48 hrs at room temp until white spots are noticeable in the plate. For me, it took 72 hrs until I noticed any growth. After that, when using the blue light and the filter above the spots in the plate, they clearly illuminated a green color. It was a fun experiment. I would like to try this DNA technique on something else to see if I can make it have the same effect.
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2nd time i buy it!
and i would buy it a third, good for beginners even though a few more detailed instructions and info would help. with the ongoing pandemic it even shipped faster than anything else on other websites (amazon lol) .
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Great experience
Fun and direct. Will be purchasing the full classes after receiving this kit. Definitely worth every. penny.
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Could be explained better.
The kit itself and instructions are great, but I don't feel as if the handbook had enough information to learn from and seemed geared more for beginners. I hoped for a more in depth explanation of what is going on in each process, not a simple overview.
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Very easy and very straightforward
Even though it was very easy and straightforward instructions were a little confusing but I got the hang of it very easy. I wish they had a video series showing me how to do it step-by-step.