Genetically Engineer Any Brewing or Baking Yeast to Fluoresce
Our goal with this kit is to begin to integrate synthetic biology and genetic design into people's everyday life. We see a future in which people are genetically designing the plants they use in their garden, eating yogurt that contains a custom bacterial strain they modified or even someday brewing using an engineered yeast strain.
Yeast is an integral part of our lives. It can used be used for brewing, baking, fermentation or as a research tool. Genetically Engineering yeast in your home seems like Science Fiction but is actually now reality. Using our kit you can make your yeast fluoresce and glow by inserting a gene from a jellyfish, the Green Fluorescent Protein(GFP). This kit comes with everything you need to engineer a Brewing Yeast we provide or your own favorite yeast that you provide. Most any yeast you can buy from a store you can engineer!
That is what makes this kit so powerful is that you can engineer laboratory yeast, yeast isolated from the environment, brewing yeast, baking yeast or even maybe yeast from your skin!
The kit uses a genetically designed extra chromosomal DNA(plasmid) that contains a form of the Green Fluorescent Protein. This protein makes it so that when you expose the engineered yeast to a "black light" or blue light and the yeast glow as seen in the picture.
Some items in this kit need to be stored in a fridge and a freezer upon you receiving them.
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This yeast and plasmid are being released through a Creative Commons Non-Commercial License we encourage you to share it. For commercial use we ask that you contact us for a license at firstname.lastname@example.org.
1 - YPD Agar containing G418
1 - 250 mL glass bottle for pouring plates
1 - 10-100uL professional lab quality variable volume adjustable pipette
1 - Box (96 count) 1-200uL Pipette Tips
14 - Petri Plates
1 - Microcentrifuge tube rack
5 - Pairs of Nitrile Gloves
5 - Inoculation Loops
1 - Plate spreader
25~ - microcentrifuge tubes
6 - 1.5mL microfuge tubes containing YPD
50mL centrifuge tube for measuring liquid volume
1 mL Yeast transformation buffer 40% PEG 8000, 200mM LiAc, 0.1mg/mL Salmon Sperm DNA
1 - Preengineered strain of Yeast Containing GFP Plasmid
1 - UV Filter paper and blue light