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Glow-in-the-Dark Bacteria Engineering Kit

Glow-in-the-Dark Bacteria Engineering Kit

$39.99
(28 reviews) Write a Review
Current Stock:
Description

Make bacteria glow green using a gene borrowed from jellyfish - then watch the results grow overnight on a petri dish.

Green Fluorescent Protein (GFP) won the Nobel Prize. Scientists use it to track genes, light up cancer cells, and visualize things invisible to the naked eye. In this kit, you'll put that exact gene into harmless E. coli - and see glowing colonies appear under blue light within 24 hours.

This is the experiment that hooks people on biology for life.

WHAT YOU'LL DO

  • Rehydrate freeze-dried bacteria and GFP plasmid DNA
  • Run a heat-shock bacterial transformation - the same technique used in real labs
  • Grow your engineered bacteria overnight on selective agar plates
  • Illuminate the results with the included blue light and UV filter

WHAT YOU'LL LEARN

  • How fluorescent proteins work and why they matter in research
  • The mechanics of bacterial transformation - how DNA gets into a cell
  • Gene expression: why some bacteria glow and others don't
  • How scientists use antibiotic selection to identify successful edits

PERFECT FOR
Total beginners. No lab experience needed. Works in a kitchen. Great for curious adults, teens, science fair projects, and classrooms.

NOTES

• All biological materials are freeze-dried and stable at room temperature for up to one month.
• Includes step-by-step transformation protocol and a guide to fluorescent proteins.

WHAT'S INCLUDED:
Biological Materials:

  • Non-pathogenic E. coli (DH5α strain) bacteria, freeze dried
    GFP plasmid containing jellyfish fluorescent gene (5 - 50uL of 20ng/uL)

Media & Reagents:

  • LB agar media
    LB selective agar (Kan 100 µg/ml)
    Transformation buffer (25mM CaCl2, 10% PEG 8000)
    LB recovery media

Lab Supplies:

  • 7 Petri plates
    Transfer pipettes
    Microcentrifuge tubes
    Test tube for measuring 50 mL
    UV Filter Sheet 
    Blue light
    Agar preparation bottle
    5 Sterile inoculation loops
    10 nitrile gloves

  

Link to protocol

**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**

 

Additional Information
Width:
17.78 (cm)
Height:
17.78 (cm)
Depth:
17.78 (cm)

Reviews

  • 5
    Straightforward and excellent for beginners

    Posted by Dory A Muller on 24th Jun 2019

    Shipping was awesome and earlier than expected. Instructions were clear and succinct. I was really satisfied with the shelf life of the bacteria and transformation mix--was worried that my delay in completing my experiments would reduce the likelihood of a successful one. Nonetheless, it worked except for the first amp tray...it would be really nice if you could include more educational components that highlights the mechanics and background of each kit item. That way, we can have a wider scope of background knowledge, making the experience that much more enjoyable.

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