Glow-in-the-Dark Bacteria Engineering Kit
$39.99
Make bacteria glow green using a gene borrowed from jellyfish - then watch the results grow overnight on a petri dish.
Green Fluorescent Protein (GFP) won the Nobel Prize. Scientists use it to track genes, light up cancer cells, and visualize things invisible to the naked eye. In this kit, you'll put that exact gene into harmless E. coli - and see glowing colonies appear under blue light within 24 hours.
This is the experiment that hooks people on biology for life.
WHAT YOU'LL DO
- Rehydrate freeze-dried bacteria and GFP plasmid DNA
- Run a heat-shock bacterial transformation - the same technique used in real labs
- Grow your engineered bacteria overnight on selective agar plates
- Illuminate the results with the included blue light and UV filter
WHAT YOU'LL LEARN
- How fluorescent proteins work and why they matter in research
- The mechanics of bacterial transformation - how DNA gets into a cell
- Gene expression: why some bacteria glow and others don't
- How scientists use antibiotic selection to identify successful edits
PERFECT FOR
Total beginners. No lab experience needed. Works in a kitchen. Great for curious adults, teens, science fair projects, and classrooms.
NOTES
• All biological materials are freeze-dried and stable at room temperature for up to one month.
• Includes step-by-step transformation protocol and a guide to fluorescent proteins.
WHAT'S INCLUDED:
Biological Materials:
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Non-pathogenic E. coli (DH5α strain) bacteria, freeze dried
GFP plasmid containing jellyfish fluorescent gene (5 - 50uL of 20ng/uL)
Media & Reagents:
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LB agar media
LB selective agar (Kan 100 µg/ml)
Transformation buffer (25mM CaCl2, 10% PEG 8000)
LB recovery media
Lab Supplies:
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7 Petri plates
Transfer pipettes
Microcentrifuge tubes
Test tube for measuring 50 mL
UV Filter Sheet
Blue light
Agar preparation bottle
5 Sterile inoculation loops
10 nitrile gloves
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
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Reviews
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Great Kit
The kit worked very well for my students despite their having to place the cells in the refrigerator post-recovery until class the next day. We got plenty of successful colonies even with the group that kept their post-recovery cells in the refrigerator for two days.
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Great product
Used it to much sucess for my 6th-grade science fair! Gave my whole class lots of wows.
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Muito bom
Adorei, atendeu todas minhas espectativas.
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Wonderful Product
This kit worked wonderfully for my students! The materials provided were perfectly suitable for completing the experiment without issue, and we had an incredible transformation efficiency without hundreds of fluorescent colonies detected. I will certainly be buying this from The ODIN again next year. So appreciative!
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GFP Kit
The kit's awesome! Special shoutout to the team for customizing the kit and providing additional support via email! Can't wait to try out the crispr kit.
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fantastic and eye-opening experiments for beginner
straightforward instructions (though it is recommended to read everything before getting started) . If doing these experiments during the cold season, have a space heater handy to speed up incubation times. One minor annoyance: not all packages are complete so good to check everything is there before getting started.
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Really fun and simple
This was a really fun and simple project I had a lot of fun doing it, it was really interesting to get to know how everything in it worked
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Really fun and simple activity
I had a lot of fun with this project and it was really interesting learning how everything worked. The user manual was simple and easy to read and very user friendly.
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Great for Novice Biohackers
Two teachers at our high school run the CHS Science Club and after watching Unnatural Selection my colleague and I decided to look into The ODIN to see what was available for our fledgling scientists/biohackers to work with. We decided to try the Genetic Design Starter Kit and the results were great. Since we usually only have 30-60 minutes once or twice a week with our group we were able to make things work. The students thoroughly enjoyed the process and learning about biohacking. After completing the procedure we did have successful transformation and saw some glowing colonies. It was great!