Engineer bacteria to glow using the famous jellyfish GFP gene.
In this experiment you will insert a fluorescent protein gene into harmless E. coli. Once expressed, the bacteria produce a bright green glow under blue light — a classic demonstration of genetic engineering.
• Introduce a GFP plasmid into bacteria
• Perform a bacterial transformation
• Grow genetically engineered fluorescent colonies
• How fluorescent proteins work
• Gene expression in bacteria
• How scientists track genes using fluorescence
Biological Materials
Non-pathogenic E. coli (DH5α strain) bacteria, freeze dried
GFP plasmid containing jellyfish fluorescent gene (5 - 50uL of 20ng/uL)
Media & Reagents
LB agar media
LB selective agar (Kan 100 µg/ml)
Transformation buffer (25mM CaCl2, 10% PEG 8000)
LB recovery media
Lab Supplies
7 Petri plates
Transfer pipettes
Microcentrifuge tubes
Test tube for measuring 50 mL
UV Filter Sheet
Blue light
Agar preparation bottle
5 Sterile inoculation loops
10 nitrile gloves
Documentation
Step-by-step bacterial transformation protocol
Guide to fluorescent proteins
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
This is the second product I’ve ordered from the Odin. The instruction manual is clear, there are online guides, and wow, I’ve learned a lot about genetics and biology!
First time - worked perfectly, even with a few human errors and extended time in between stages. Used with 7th graders.
The kit was well packaged and the directions were thorough. My son and his lab partner were able to complete this for their science fair project. They were featured in our local news! I will be purchasing more for my students.
I ordered the Genetic Design Starter Kit for my grandson’s science fair project. It was easy enough to follow for me to help him. He is in 7th grade and very interested in cutting edge science.
Vraiment super
Yes! I have created life! The instructions were a little ambiguous if it is your first time and culturing at room temp takes more like 4 days. But, man, creating life is awesome. Now I know what it is to be a mother.
Works as advertised, read through the experiment a few times before you get started. Already ordered my Bioengineering 101, can't wait!!!
Had lots of fun doing this and got a lot of hands on experience. I would suggest that the kit come with extra inoculation loops because I almost ran out. I would love to see more kits like this or possibly some empty backbones to design your own plasmid for the more advanced users. Also tip to those who have contamination issues, performing parts of the experiment, like streaking, in a sandwich bag or oven bag is a good way to keep things sterile, at least from my experience.
I love the new and improved jellyfish genetics kit! I ordered one last year to use in my 7th grade science classroom. The issues I had with the previous kit, such as scattered materials and convoluted instructions, seem to be fixed with the new iteration of the kit. I love that it comes with links to instructional videos and materials. My kids have a blast doing their genetic design projects each year!