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Glow-in-the-Dark Bacteria Engineering Kit

$39.99
(27 reviews) Write a Review
Current Stock:

 

Engineer bacteria to glow using the famous jellyfish GFP gene.

 

In this experiment you will insert a fluorescent protein gene into harmless E. coli. Once expressed, the bacteria produce a bright green glow under blue light — a classic demonstration of genetic engineering.

 

What You'll Do

 

• Introduce a GFP plasmid into bacteria
• Perform a bacterial transformation
• Grow genetically engineered fluorescent colonies

 

What You'll Learn

 

• How fluorescent proteins work
• Gene expression in bacteria
• How scientists track genes using fluorescence

 

Perfect For: Students, classrooms, and first-time genetic engineering experiments.

 

What’s Included:

 

Biological Materials

 

  • Non-pathogenic E. coli (DH5α strain) bacteria, freeze dried

  • GFP plasmid containing jellyfish fluorescent gene (5 - 50uL of 20ng/uL)

 

Media & Reagents

 

  • LB agar media

  • LB selective agar (Kan 100 µg/ml)

  • Transformation buffer (25mM CaCl2, 10% PEG 8000)

  • LB recovery media

 

Lab Supplies

 

  • 7 Petri plates

  • Transfer pipettes

  • Microcentrifuge tubes

  • Test tube for measuring 50 mL

  • UV Filter Sheet 

  • Blue light

  • Agar preparation bottle

  • 5 Sterile inoculation loops

  • 10 nitrile gloves

 

Documentation

 

  • Step-by-step bacterial transformation protocol

  • Guide to fluorescent proteins

 

Link to protocol

**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**

 

Width:
17.78 (cm)
Height:
17.78 (cm)
Depth:
17.78 (cm)

Reviews

  • 5
    Fantastic product!

    Posted by Jeff Shupe on 6th Jun 2022

    This is the second product I’ve ordered from the Odin. The instruction manual is clear, there are online guides, and wow, I’ve learned a lot about genetics and biology!

  • 5
    Genetic Design Starter Kit - Glowing Jellyfish Bacteria

    Posted by SANDRA SIMONDS on 12th May 2022

    First time - worked perfectly, even with a few human errors and extended time in between stages. Used with 7th graders.

  • 5
    Fantastic!

    Posted by Loyal Anderson on 29th Apr 2022

    The kit was well packaged and the directions were thorough. My son and his lab partner were able to complete this for their science fair project. They were featured in our local news! I will be purchasing more for my students.

  • 5
    Great Science Experiment

    Posted by Unknown on 18th Dec 2021

    I ordered the Genetic Design Starter Kit for my grandson’s science fair project. It was easy enough to follow for me to help him. He is in 7th grade and very interested in cutting edge science.

  • 5
    bonne expérience

    Posted by Unknown on 18th Aug 2021

    Vraiment super

  • 4
    Awesome

    Posted by Edward VanBuskirk on 9th May 2021

    Yes! I have created life! The instructions were a little ambiguous if it is your first time and culturing at room temp takes more like 4 days. But, man, creating life is awesome. Now I know what it is to be a mother.

  • 5
    Amazing!!!

    Posted by James Krushlucki on 30th Jan 2021

    Works as advertised, read through the experiment a few times before you get started. Already ordered my Bioengineering 101, can't wait!!!

  • 5
    Worth it

    Posted by Unknown on 26th Jan 2021

    Had lots of fun doing this and got a lot of hands on experience. I would suggest that the kit come with extra inoculation loops because I almost ran out. I would love to see more kits like this or possibly some empty backbones to design your own plasmid for the more advanced users. Also tip to those who have contamination issues, performing parts of the experiment, like streaking, in a sandwich bag or oven bag is a good way to keep things sterile, at least from my experience.

  • 5
    New and Improved

    Posted by Whitney McBrayer on 22nd Jan 2021

    I love the new and improved jellyfish genetics kit! I ordered one last year to use in my 7th grade science classroom. The issues I had with the previous kit, such as scattered materials and convoluted instructions, seem to be fixed with the new iteration of the kit. I love that it comes with links to instructional videos and materials. My kids have a blast doing their genetic design projects each year!