Edit Bacteria with CRISPR
$59.00 - $129.00
This kit comes with an example experiment that teaches you many molecular biology and gene engineering techniques.
However, if you are looking to use this kit to get a deeper understanding of bioengineering we recommend that you purchase our Learn Bioengineering Bundle which includes this kit as part of it.
We Fulfill and Ship in 2-3 days in the US. Overnight is not required and neither are ice packs.
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering? This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, gRNA and Template DNA template for an example experiment.
Includes example experiment to make a genome mutation(K43T) to the rpsL gene changing the 43rd amino acid, a Lysine(K) to a Threonine(T) thereby allowing the bacteria to survive on Strep media which would normal prevent its growth.
Kit contains everything you need for around 5 experiments or more
- LB Agar
- LB Strep/Kan/Arabinose Agar
- Glass bottle for pouring plates
- Non-pathogenic E. coli DH5a bacteria
- Inoculation Loops
- 10-100uL variable volume adjustable pipette(1uL increments)
- Box of 96 Pipette Tips
- 14 Petri Plates
- Microcentrifuge tube rack
- Nitrile Gloves
- Microcentrifuge tubes
- 50mL Tube for measuring
- Bacterial transformation buffer 25mM CalCl2, 20% PEG 8000
- LB Media for transformation recovery
- Cas9 plasmid
- gRNA plasmid
- Template DNA
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
Some items in this kit need to be stored in a fridge and a freezer upon you receiving them.
Take a look at our Experiment Protocol
Access plasmid maps.
Ordering the Refill/Classroom kit? Here's what it includes:
- Cas9 plasmid
- gRNA plasmid
- Template DNA
- Strep/Kan/Arabinose Agar Media
- LB Agar Media
- LB Media
- 20 microcentrifuge tubes
- 14 plates
- Non-pathogenic DH5a E. coli bacteria
- Inoculation loops
- Bacterial Transformation Buffer
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
*The REFILL KIT kit does not contain a pipette, pipette tips and a glass bottle for making media. These must be user supplied.
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Reviews
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It works!
I have read a lot about CRISPR, but I didn't know it could be this easy. The kit arrived with everything I needed and the instructions were thorough and easy to understand.