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DIY Bacterial Gene Engineering CRISPR Kit
$85.00 - $179.00
If you are looking to use this kit to learn molecular biology and genetic engineering we instead recommend that you purchase our Learn Bioengineering Class which includes this kit as part of it. You can select payment plan on check out for the class to split the cost into 4 monthly payments of $80 each.
We Fulfill and Ship in 2-3 days in the US. Overnight is not required and neither are ice packs.
Due to the overwhelming number of emails we will not respond to emails asking when your item will be shipped. Understand we are doing our best to get it to you.
Comes with an example experiment that teaches you many molecular biology and gene engineering techniques.
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering? This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, gRNA and Template DNA template for an example experiment.
Includes example experiment to make a genome mutation(K43T) to the rpsL gene changing the 43rd amino acid, a Lysine(K) to a Threonine(T) thereby allowing the bacteria to survive on Strep media which would normal prevent its growth.
Kit contains enough materials for around 5 experiments or more
- LB Agar
- LB Strep/Kan/Arabinose Agar
- Glass bottle for pouring plates
- Non-pathogenic E. coli DH5a bacteria
- Inoculation Loops
- 10-100uL variable volume adjustable pipette(1uL increments)
- Box of 96 Pipette Tips
- 14 Petri Plates
- Microcentrifuge tube rack
- Nitrile Gloves
- Microcentrifuge tubes
- 50mL Tube for measuring
- Bacterial transformation buffer 25mM CalCl2, 20% PEG 8000
- LB Media for transformation recovery
- Cas9 plasmid
- gRNA plasmid
- Template DNA
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
Access plasmid maps here.
Some items in this kit need to be stored in a fridge and a freezer upon you receiving them.
Ordering the Refill/Classroom kit? Here's what it includes:
Kit contents
- Cas9 plasmid
- gRNA plasmid
- Template DNA
- Strep/Kan/Arabinose Agar Media
- LB Agar Media
- LB Media
- 20 microcentrifuge tubes
- 14 plates
- Non-pathogenic DH5a E. coli bacteria
- Inoculation loops
- Bacterial Transformation Buffer
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
*This kit does not contain pipette, pipette tips and glass bottle for making media. These must be user supplied.
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Reviews
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Amazing!
The kit was easy and very interesting. The only issue was that it could have included more inoculation loops, although there is enough to carry out one experiment, I don't think that there is enough for two or more.
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Great Kit！
It Works!
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Best CRISPR Kit on the market
I am a high school student who purchased this kit because I had a passion to learn more about biohacking at home, and be involved with genetic engineering independently. This kit was the perfect stepping stool to give me first hand info on CRISPR and the whole process is so easy to understand, everything you need is right there in the kit, just be careful NOT to freeze your E. coli like I did :(
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Excellent Introduction to technical details of CRISPR
Rand three trials so far and every one was successful.
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Works, but Protocol is Confusing
Shipping took a while, but the kit included all materials including two stickers with the ODIN logo. The experiment was a success - we got a lot of growth on the plates. The only frustration was the protocol was confusing and wordy. I ended up rewriting it so it was more straightforward without the protocols and walkthroughs in the middle of the handout. Otherwise, great kit! Ordered the refill to introduce to the class next term.
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Bio Art
I was able to successfully complete this experiment. I am now working on sequencing the before and after bacteria and using the resulting data to drive one of my videos. Thank you Odin.
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Satisfied Curious Newbie!
Fascinated by CRISPR, I wanted to learn about it. This Kit was impressive - thoughtful, well organized, fully equipped with extra supplies for mess ups, affordable and backed by technical information. Just what I wanted. Thanks so much!!
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Best CRISPR kit on the market
Okay, it's also kind of the only CRISPR kit on the market right now. But it's nevertheless a wonderful and very simple kit.
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It works!
I have read a lot about CRISPR, but I didn't know it could be this easy. The kit arrived with everything I needed and the instructions were thorough and easy to understand.