CRISPR Bacteria Gene Editing Kit
$59.00 - $129.00
Use the same gene-editing tool behind Nobel Prize–winning research to rewrite living DNA - and read the results on an agar plate.
CRISPR-Cas9 changed biology. It's how scientists are developing cures for genetic diseases, engineering drought-resistant crops, and rewriting genomes with a precision that wasn't possible a decade ago. In this kit, you'll use the real thing.
You'll introduce CRISPR components into harmless E. coli, direct Cas9 to cut a specific gene, and repair it with a custom mutation that gives the bacteria antibiotic resistance. The colonies that survive on your selection plates are the ones you successfully edited.
WHAT YOU'LL DO
- Transform bacteria with Cas9, guide RNA, and a DNA repair template
- Target a specific genomic location using guide RNA
- Grow edited and unedited bacteria side by side on antibiotic plates
- Confirm successful edits by comparing colony survival rates
WHAT YOU'LL LEARN
- How CRISPR-Cas9 finds and cuts specific DNA sequences
- How cells repair DNA breaks - and how that repair can be guided
- The role of plasmids in delivering genetic tools into cells
- Why antibiotic selection is a cornerstone of genetic engineering
PERFECT FOR
Students, biohackers, science educators, and anyone who has wondered how CRISPR actually works in practice. No prior lab experience required.
NOTES
• All biological materials are freeze-dried and stable at room temperature for up to one month. Some reagents require refrigeration upon arrival.
• Includes step-by-step experiment guide, CRISPR theory booklet, and plasmid maps.
• The $59 base kit and $129 classroom kit include the same experiment - the classroom version scales supplies for groups of 10+.
WHAT'S INCLUDED:
Biological Materials
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Non-pathogenic E. coli (DH5α strain), Freeze Dried
Cas9 expression plasmid
Guide RNA plasmid
DNA repair template
Media & Reagents
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LB agar media
Selective antibiotic agar media (LB Strep/Kan/Arabinose)
Transformation buffer (25mM CalCl2, 20% PEG 8000)
LB recovery media
Lab Supplies
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14 petri plates
Adjustable micropipette (10–100 µL)
Pipette tips (box of 96)
Microcentrifuge tubes
Tube rack
Inoculation loops
Nitrile gloves
Agar preparation glass bottle
Documentation
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Step-by-step experiment guide
CRISPR theory and protocol booklet
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
Take a look at our Experiment Protocol
Access plasmid maps.
Some items in this kit need to be stored in a fridge and a freezer upon you receiving them.
We Fulfill and Ship in 2-3 days in the US. Overnight is not required and neither are ice packs.
*The REFILL KIT kit does not contain a pipette, pipette tips and a glass bottle for making media. These must be user supplied.
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Reviews
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Very cool
Directions are a bit vague - yet I still succeed with zero experience! Highly recommend, very cool this is offered to the public.
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Gift for my GF
This is a gift for my GF, she study for become a biotechnologist and she is very happy when recive this kit. Tnx for all ODIN Sorry for my English but I come from Italy:)
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A fantastic product with the UK shipping
The Odin CRISPR/Cas9 bacterial kit is a creative and effective way for everyone who wants to learn biomedical engineering or biohacking. It has everything you need to perform a simple experiment and learn how to use basic laboratory equipment. Also, instructions are written in a very clear and engaging way and it has been shipped to the UK with no issues! Thanks the Odin!
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Just what the doctor ordered
Great product
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Great introduction to Cas9 DNA editing.
Just finished growing my own DNA edited bacteria in my home office. I come from a microelectronics background so working with precision tools and small amounts of material were natural. Succeeding the first time working with engineering DNA was a true rush. This was a lot of science fun and I can't wait to try another kit from Odin.
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Great product!
The kit was easy to use and a great way to get experience with genetic engineering. There were just a few times where the directions could have a bit more detail, however, they were still sufficient and generally very clear.
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Contains absolutely everything
The kit is very complete, which is useful for those with no other lab equipment or resources. It is good to see that a perishables replacement kit is now available. I would also like to have the protocols available electronically as a PDF.
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Wonderful introduction to CRISPR
As a retired professional in a different science field and having little or no micro/biology training I learned quite a bit with this kit and online tutorials. The folks, especially Peter at "The Odin" were very helpful and professional responding to all my emails with detailed explanations and guidance. I strongly recommend this kit for folks who would like to learn and explore some broad understanding of this astounding technology.
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Great Product
Great Product! A really fun experiment and great introduction to the technology. I would recommend this product to anyone wanting to learn the basics or anyone that once to be a scientist for a weekend.