DIY Bacterial Gene Engineering CRISPR Kit
PLEASE MAKE REPLACEMENT KIT!!!
Posted by Unknown on 22nd May 2019
Great kit, worked well!
Please consider making a cheaper replacement kit with just the bacteria, tranformation mix, LB agar, LB Strep/Kan/Ara Agar, LB media, Cas9 plasmid, gRNA, and template DNA. Many of us who want to repeat this experiment in the classroom yearly already have the other materials in our labs and need to watch our budgets.
I will buy again!
Exactly as promised.
Posted by Celesta Bargatze on 3rd May 2019
I received the kit very quickly, within the week I think. Everything was as promised and support was good as well. It worked perfectly and the instructions were helpful and easy to follow. I would definitely recommend ordering from them and will use The Odin for future classroom supply needs.
Posted by George Trevor on 4th Apr 2019
The kit was easy and very interesting. The only issue was that it could have included more inoculation loops, although there is enough to carry out one experiment, I don't think that there is enough for two or more.
Posted by YANG JIAJIANG on 26th Mar 2019
Best CRISPR Kit on the market
Posted by Camille Lienau on 5th Apr 2018
I am a high school student who purchased this kit because I had a passion to learn more about biohacking at home, and be involved with genetic engineering independently. This kit was the perfect stepping stool to give me first hand info on CRISPR and the whole process is so easy to understand, everything you need is right there in the kit, just be careful NOT to freeze your E. coli like I did :(
Excellent Introduction to technical details of CRISPR
Posted by Arthur on 14th Nov 2017
Rand three trials so far and every one was successful.
Works, but Protocol is Confusing
Posted by Sarah on 9th Nov 2017
Shipping took a while, but the kit included all materials including two stickers with the ODIN logo. The experiment was a success - we got a lot of growth on the plates. The only frustration was the protocol was confusing and wordy. I ended up rewriting it so it was more straightforward without the protocols and walkthroughs in the middle of the handout. Otherwise, great kit! Ordered the refill to introduce to the class next term.
Posted by James Pricer on 22nd Aug 2017
I was able to successfully complete this experiment. I am now working on sequencing the before and after bacteria and using the resulting data to drive one of my videos. Thank you Odin.
Satisfied Curious Newbie!
Posted by Mike Russo on 5th Feb 2017
Fascinated by CRISPR, I wanted to learn about it. This Kit was impressive - thoughtful, well organized, fully equipped with extra supplies for mess ups, affordable and backed by technical information. Just what I wanted. Thanks so much!!
Best CRISPR kit on the market
Posted by Jake on 17th Aug 2016
Okay, it's also kind of the only CRISPR kit on the market right now. But it's nevertheless a wonderful and very simple kit.
If you are looking to use this kit to learn molecular biology and genetic engineering we instead recommend that you purchase our Learn Bioengineering Class which includes this kit as part of it. You can select payment plan on check out for the class to split the cost into 4 monthly payments of $80 each.
We Fulfill and Ship in 2-3 days in the US. Overnight is not required and neither are ice packs.
Due to the overwhelming number of emails we will not respond to emails asking when your item will be shipped. Understand we are doing our best to get it to you.
Comes with an example experiment that teaches you many molecular biology and gene engineering techniques.
Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering? This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, gRNA and Template DNA template for an example experiment.
Includes example experiment to make a genome mutation(K43T) to the rpsL gene changing the 43rd amino acid, a Lysine(K) to a Threonine(T) thereby allowing the bacteria to survive on Strep media which would normal prevent its growth.
Kit contains enough materials for around 5 experiments or more
- LB Agar
- LB Strep/Kan/Arabinose Agar
- Glass bottle for pouring plates
- Non-pathogenic E. coli DH5a bacteria
- Inoculation Loops/Plate Spreader
- 10-100uL variable volume adjustable pipette(1uL increments)
- Box of 96 Pipette Tips
- 14 Petri Plates
- Microcentrifuge tube rack
- Nitrile Gloves
- Microcentrifuge tubes
- 50mL Tube for measuring
- Bacterial transformation buffer 25mM CalCl2, 20% PEG 8000
- LB Media for transformation recovery
- Cas9 plasmid
- gRNA plasmid
- Template DNA
You can find the plasmid DNA sequences
Some items in this kit need to be stored in a fridge and a freezer upon you receiving them.