CRISPR Bacteria Gene Editing Kit

Use the same gene-editing tool behind Nobel Prize–winning research to rewrite living DNA - and read the results on an agar plate.

CRISPR-Cas9 changed biology. It's how scientists are developing cures for genetic diseases, engineering drought-resistant crops, and rewriting genomes with a precision that wasn't possible a decade ago. In this kit, you'll use the real thing.

You'll introduce CRISPR components into harmless E. coli, direct Cas9 to cut a specific gene, and repair it with a custom mutation that gives the bacteria antibiotic resistance. The colonies that survive on your selection plates are the ones you successfully edited.

WHAT YOU'LL DO

• Transform bacteria with Cas9, guide RNA, and a DNA repair template
• Target a specific genomic location using guide RNA
• Grow edited and unedited bacteria side by side on antibiotic plates
• Confirm successful edits by comparing colony survival rates

WHAT YOU'LL LEARN

• How CRISPR-Cas9 finds and cuts specific DNA sequences
• How cells repair DNA breaks - and how that repair can be guided
• The role of plasmids in delivering genetic tools into cells
• Why antibiotic selection is a cornerstone of genetic engineering

PERFECT FOR
Students, biohackers, science educators, and anyone who has wondered how CRISPR actually works in practice. No prior lab experience required.

NOTES

• All biological materials are freeze-dried and stable at room temperature for up to one month. Some reagents require refrigeration upon arrival.

• Includes step-by-step experiment guide, CRISPR theory booklet, and plasmid maps.

• The $59 base kit and $129 classroom kit include the same experiment - the classroom version scales supplies for groups of 10+.

WHAT'S INCLUDED:
Biological Materials

• Non-pathogenic E. coli (DH5α strain), Freeze Dried
  Cas9 expression plasmid
  Guide RNA plasmid
  DNA repair template

Media & Reagents

• LB agar media
  Selective antibiotic agar media (LB Strep/Kan/Arabinose)
  Transformation buffer (25mM CalCl2, 20% PEG 8000)
  LB recovery media

Lab Supplies

• 14 petri plates
  Adjustable micropipette (10–100 µL)
  Pipette tips (box of 96)
  Microcentrifuge tubes
  Tube rack
  Inoculation loops
  Nitrile gloves
  Agar preparation glass bottle

Documentation

• Step-by-step experiment guide
  CRISPR theory and protocol booklet

**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**

Take a look at our Experiment Protocol

Access plasmid maps.

Some items in this kit need to be stored in a fridge and a freezer upon you receiving them.
We Fulfill and Ship in 2-3 days in the US. Overnight is not required and neither are ice packs.

*The REFILL KIT kit does not contain a pipette, pipette tips and a glass bottle for making media. These must be user supplied.