pDusk and pDawn - Light controlled fluorescent protein expression
Posted by Lukas Kingry on 18th Oct 2016
Good quality, fair price, fast shipping. always my pleasure ;)
great piece of synthetic biology
Posted by David on 12th Aug 2016
I got both pDusk and Ddawn, I'm working on cloning in my own gene of interest to get light controlled expression in bacteria. They take a while for expression to change but there are so many cool possibilities with these guys.
These plasmid were created by my good friends Robert Ohlendorf and Andreas Moglich at Humboldt-Universität in Berlin, Germany. Plasmids come pre transformed in BL21 strains of bacteria.
These plasmids are awesome!!!
When in bacteria pDusk expresses DsRed fluorescent protein in the dark and does not in the light, while pDawn expresses DsRed in the light and does not in the dark. The genes that control light activation are constitutively active so these plasmids can be used in most bacterial strains but work best in BL21 and other overexpression strains! These plasmids are Kanamycin resistant. Make your own gene light activated by cloning it between the 5' NdeI and 3' HindIII restriction sites.
For more information read the paper Here
The guide/tutorial for the kit that contains these is Here
Plasmids come ready to use in E. coli BL21 strain of bacteria, just put in a culture containing Kanamycin. Wait till the culture becomes cloudy before exposing to light. Bacteria take 5-10 hours before visible color appears or disappears.