This class is pre-recorded but a teacher will be available to answer your questions.
In this class you will learn how to culture, grow and genetically modify HEK 293 human cells in culture. This is a new version of our human cells kit that puts the lacZ gene into the human cells so that they create a blue color change. No experience needed but some experience is preferred. We will not be teaching basic skills like pipetting which are needed for this class.
Upon registering you will receive an email invite to the online classroom.
Can do international shipping but because of time requirements the cost is generally around $300-$400. Shipping to Canada is only around $100 extra.
If you can't afford the full cost of the class upfront, use our payment plan on checkout to split the price into 4 monthly payments.
This class is 4 weeks long and will cover
Culturing Human Cells
Maintenance and Splitting Passaging of Human Cells
Items Included (WARNING You cannot do this class without pipettes and an inverted microscope!!!)
1 - 25cm Cell Culture Treated Flasks with HEK293 filled with L15 + 10% Fetal Bovine Serum + Amp/Strep/Gent
- 4 - 25cm Cell Culture Treated Flasks
100mL of L15 + 10% Fetal Bovine Serum + Amp/Strep/Gent (Media for growing cells)
10 - Syringes and Filters
4 - 12 well plates
20 pairs nitrile gloves
50mL Trypsin EDTA Solution
100uL G418 200mg/mL
50 Mircocentrifuge tubes
10 - 50mL tubes
10 - 10mL tubes
4 sided tube rack
#Not provided is > 90% Isopropyl Alcohol for cleaning
*Microscope is monocular inverted microscope with 4x, 10x and 20x objectives and LED illumination. 3MP digital camera interfaces with AMScope Software.
*IN ORDER TO PROVIDE THE BEST QUALITY HUMAN CELLS, SHIPPING TIME MAY VARY*
Got the kit, including a healthy-looking colony of cells. Did the first prep and had to leave for a business trip. Returned one week later to a random bunch of panicked cells clinging to the walls. I will have to redo this but not your fault, and I love learning the process.
How else are you going to learn to work with Human Cell Culture? You're not. This is a very unique offering you won't find anywhere else. Kit contains everything you need + great support from the ODIN team. Highly recommend.
Great prices, faster than expected delivery, and perfect customer service. Can't wait for the PSE kit.
I have been very pleased with my investment in this course and the support I have received so far. Josiah is great at taking his years of experience in this field and distilling it to some functional procedures that can help advanced beginner or intermediate students to gain working procedural knowledge of one of the most important areas of knowledge in biology - the very code of life itself. This will play an ever increasingly large role in the future of medicine, agriculture and what it means to be human. Josiah has worked closely with others and developed a course around somewhat unconventional methods that can allow people to do this work at home with a minimum of equipment that would ordinarily be prohibitively expensive or require specialized skills to build. This course is ideally suited for folks who have some basic working knowledge of molecular biology procedures and can be self-directed and disciplined in their approach to this work. This is not spoon-feeding, and you may encounter issues with contamination or culture growth that - while Dr. Zayner may be able to help you with, you should expect to do teh hard work yourself to troubleshoot and move forward. The ODIN graciously allows for these possibilities and will replace your initial cultures if they fail due to contamination, freezing during shipping, etc. All of this makes this an excellent course if you're serious about learning. The ODIN has other courses that can bring you up to speed on basic lab procedures such as pipette use, making and streaking plates, etc., that - if you're not already skilled in these areas - will bring you up to speed. Highly recommended.
This course is a one-of-kind opportunity for anyone to learn and actually perform Human Cell culture and engineering through concise and straightforward remote instruction. If it simply delivered on the its premise as advertised (which it does) it would be an excellent value. However, I found that it exceeded expectations in the quality of instruction delivered. Furthermore, the provider's willingness to assist learners and responsiveness to questions is beyond what I have observed in online courses offered through more traditional institutions.
This is a weekly online class, using materials that the odin sends you. The class walks though each step, and it's pretty simple. The class involves tissue culture, which is much less forgiving than E. coli cultures !