Want to really know what this whole CRISPR thing is about? Why it could revolutionize genetic engineering? This kit includes everything you need to make precision genome edits in bacteria at home including Cas9, gRNA and Template DNA template for an example experiment.
Includes example experiment to make a genome mutation(K43T) to the rpsL gene changing the 43rd amino acid, a Lysine(K) to a Threonine(T) thereby allowing the bacteria to survive on Strep media which would normal prevent its growth.
Kit contains everything you need and enough materials for 3 experiments!!!
1 - Glass bottle
7 - Petri Plates
5 - Inoculation loops
1 - bag of 1.5mL microcentrifuge
10 - blue nitrile gloves
9 - disposable pipettes
1 - bag of CRISPR Media
1 - 50mL Centrifuge tube for measuring
1 - tube of LB Agar
1 - tube of LB Agar/Strep/Kan/Arabinose
5 - microcentrifuge tubes filled with LB Broth
5 - 100uL of Bacteria Transformation Buffer
1 - Starter CRISPR Bacteria Protocol Packet
Perishables (place into bag with store 20°C sticker):
3 - Freeze Dried CAS9/gRNA/Template tubes
2 - Freeze dried DH5α tubes
1 - Sterile Water tube
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**