This kit teaches you how to insert a Jellyfish gene, Green Fluorescent Protein(GFP), into bacteria. This allows the bacteria to glow green when you shine light onto them.
Kit comes with everything you need and instructions
Enough for 5 experiments.
LB Kan Agar (Kan 100 µg/ml)
250 mL glass bottle for pouring plates
Disposable transfer pipettes
7- Petri Plates
5 - Inoculation Loops
6 - 1.5mL micro centrifuge tubes containing LB broth
50mL centrifuge tube for measuring liquid volume
5 - 0.1 mL bacterial transformation buffer (25mM CaCl2, 10% PEG 8000)
UV Filter Sheet and blue light (to see the glow)
Non-pathogenic E. coli bacteria
5 - 50uL of 20ng/uL GFP plasmid (freeze dried)
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
The manual says that it could take 24 to 48 hrs at room temp until white spots are noticeable in the plate. For me, it took 72 hrs until I noticed any growth. After that, when using the blue light and the filter above the spots in the plate, they clearly illuminated a green color. It was a fun experiment. I would like to try this DNA technique on something else to see if I can make it have the same effect.
and i would buy it a third, good for beginners even though a few more detailed instructions and info would help. with the ongoing pandemic it even shipped faster than anything else on other websites (amazon lol) .
Fun and direct. Will be purchasing the full classes after receiving this kit. Definitely worth every. penny.
The kit itself and instructions are great, but I don't feel as if the handbook had enough information to learn from and seemed geared more for beginners. I hoped for a more in depth explanation of what is going on in each process, not a simple overview.
Even though it was very easy and straightforward instructions were a little confusing but I got the hang of it very easy. I wish they had a video series showing me how to do it step-by-step.
Shipping was awesome and earlier than expected. Instructions were clear and succinct. I was really satisfied with the shelf life of the bacteria and transformation mix--was worried that my delay in completing my experiments would reduce the likelihood of a successful one. Nonetheless, it worked except for the first amp tray...it would be really nice if you could include more educational components that highlights the mechanics and background of each kit item. That way, we can have a wider scope of background knowledge, making the experience that much more enjoyable.