This kit teaches you how to insert a Jellyfish gene, Green Fluorescent Protein(GFP), into bacteria. This allows the bacteria to glow green when you shine light onto them.
Kit comes with everything you need and instructions
Enough for 5 experiments.
LB Kan Agar (Kan 100 µg/ml)
250 mL glass bottle for pouring plates
Disposable transfer pipettes
7- Petri Plates
5 - Inoculation Loops
6 - 1.5mL micro centrifuge tubes containing LB broth
50mL centrifuge tube for measuring liquid volume
5 - 0.1 mL bacterial transformation buffer (25mM CaCl2, 10% PEG 8000)
UV Filter Sheet and blue light (to see the glow)
Non-pathogenic E. coli bacteria
5 - 50uL of 20ng/uL GFP plasmid (freeze dried)
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
Had lots of fun doing this and got a lot of hands on experience. I would suggest that the kit come with extra inoculation loops because I almost ran out. I would love to see more kits like this or possibly some empty backbones to design your own plasmid for the more advanced users. Also tip to those who have contamination issues, performing parts of the experiment, like streaking, in a sandwich bag or oven bag is a good way to keep things sterile, at least from my experience.
I love the new and improved jellyfish genetics kit! I ordered one last year to use in my 7th grade science classroom. The issues I had with the previous kit, such as scattered materials and convoluted instructions, seem to be fixed with the new iteration of the kit. I love that it comes with links to instructional videos and materials. My kids have a blast doing their genetic design projects each year!
Messed up what bacteria to grow at the start so re-did and had success. The folks at Odin rock, love the vids and weekly updates, I'll be back for more.
This kit does require a little concentration and I found that things took a little longer than described, so...patience. Doing this kit force me to learn A LOT about how genes work and this is what I wanted. It's pretty simple but requires a number of steps. I'd recommend giving things a couple days to happen. It took a few days to get my bacteria to grow etc. But, hey, what's the hurry. Be careful who you about this. Some people have have very strong opinions about anything like this and they think you're some sort of evil scientist just for trying to learn something.
My 10 yr old son and I did this as a fun summer activity. The steps were clearly spelled out. However, I wish the instructions were a bit more detailed. He would have learned more if explanations about each "ingredient" were given, including details about the different types of agar and why they matter.
The manual says that it could take 24 to 48 hrs at room temp until white spots are noticeable in the plate. For me, it took 72 hrs until I noticed any growth. After that, when using the blue light and the filter above the spots in the plate, they clearly illuminated a green color. It was a fun experiment. I would like to try this DNA technique on something else to see if I can make it have the same effect.
and i would buy it a third, good for beginners even though a few more detailed instructions and info would help. with the ongoing pandemic it even shipped faster than anything else on other websites (amazon lol) .
Fun and direct. Will be purchasing the full classes after receiving this kit. Definitely worth every. penny.
The kit itself and instructions are great, but I don't feel as if the handbook had enough information to learn from and seemed geared more for beginners. I hoped for a more in depth explanation of what is going on in each process, not a simple overview.