Plasmid pJE202 also known as pVIB is an amp resistant plasmid that expresses the Lux operon genes from Vibrio fischeri. Originally from http://jb.asm.org/content/164/1/45.full.pdf
Plasmid comes already in E. coli DH10B strain of bacteria for immediate function. You can purify the plasmid from this strain if you want only the plasmid.
Just inoculate a culture with the bacteria and grow them at 30C or below and then take the plate or culture in a dark room to see them bioluminescence.
If you are thinking about using this to grow bioluminescent bacteria but have no experience with this please purchase this kit instead as the bacteria require certain chemicals to function and grow properly.
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
very good product
I ordered this product and I'm very happy with the results. Plated them on agar with ampicilline and after 3 days I saw the bioluminesce. Very good product, delivers what they say.
Very interesting, thanks Odin! thank you for products
That blue light
Very interesting, just beware you really do need to grow it under cool conditions two fails because too hot but third attempt in AC room BINGO, interesting how rapid the flash is when you shake it, thanks Odin!
A glowing result
every thing works as expected and was well packed, i got it in the uk pretty quickly. i got them as i want to see wot there glow looks like under a microscope and who doesnt want a biological night lite
Culture still glow at one and half year after purchasing...
I bought this bacteria at Feb. 2015 for one of my project. Josiah is such a nice guy who helped me a lot for culturing this bacteria. Now I have another project need to use them, take it out from freezer and streak on the plate, they still glow fine.
one of my favorite plasmids
Arrived exactly as described. We used this in my daughters 5 grade science project. She was a very happy girl.