pJE202/pVIB to Create Bioluminescent Bacteria
That blue light
Posted by Sean Cross on 14th Nov 2019
Very interesting, just beware you really do need to grow it under cool conditions two fails because too hot but third attempt in AC room BINGO, interesting how rapid the flash is when you shake it, thanks Odin!
A glowing result
Posted by Ryan boyer on 24th Oct 2018
every thing works as expected and was well packed, i got it in the uk pretty quickly. i got them as i want to see wot there glow looks like under a microscope and who doesnt want a biological night lite
Culture still glow at one and half year after purchasing...
Posted by Emma on 27th Sep 2016
I bought this bacteria at Feb. 2015 for one of my project. Josiah is such a nice guy who helped me a lot for culturing this bacteria. Now I have another project need to use them, take it out from freezer and streak on the plate, they still glow fine.
one of my favorite plasmids
Posted by David on 12th Aug 2016
Arrived exactly as described. We used this in my daughters 5 grade science project. She was a very happy girl.
Plasmid pJE202 also known as pVIB is an ampicillin resistant plasmid that expresses the Lux operon genes from Vibrio fischeri. Originally from http://jb.asm.org/content/164/1/45.full.pdf
Plasmid comes already in E. coli BL21 strain of bacteria for immediate function. You can purify the plasmid from this strain if you want only the plasmid.
Just innoculate a culture with the bacteria and grow them at 30C or below and then take the plate or culture in a dark room to see them bioluminescience.
If you are thinking about using this to grow bioluminescent bacteria but have no experience with this please purchase this kit instead as the bacteria require certain chemicals to function and grow properly.