This dye is a pre-mixed loading buffer which contains a combination of two dyes, Dye 1 (pink/red) and Dye 2 (blue). The red dye serves as the tracking dye for both agarose and non-denaturing polyacrylamide gel electrophoresis. The two dyes separate upon gel electrophoresis; the red band is the major indicator and migrates similarly to Bromophenol Blue on agarose gels. Specifically chosen, this dye does not leave a shadow under UV light. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. The dye also contains Ficoll, which creates brighter and tighter bands when compared to glycerol loading dyes.
1X Buffer Components
0.02% Dye 1
0.001% Dye 2
Quality Assurance Statement
- This dye has been assayed for Non-Specific DNase Activity (16 hour), Exonuclease Activity (Radioactivity Release), Endonuclease Activity (Nicking) and RNase Activity (Extended Digestion).
The first loading dye I ever used, works as described, I see the pigment separation which is a useful indicator. I mix 5ul loading dye drops with 10ul PCR product on a microscope slide, then pipette the mix into the well. I used it in conjunction with the safe gel stain sold here.
I always add 3ul of this per 10ul of PCR products or plasmid extraction . Its probably excessive, but anytime there is DNA the band is present.
This was the first loading dye I've ever purchased - I'm new to PCR/gel electrophoresis, and it worked
Works as advertised. I get nice bands when I run with the gel-green like dye also sold by the ODIN.