Engineer brewing or baking yeast to produce fluorescent proteins.
Using plasmid DNA and yeast transformation techniques, you will introduce a gene encoding a fluorescent protein into yeast cells. Once expressed, colonies glow under blue light, demonstrating successful genetic engineering.
What You'll Do
• Transform yeast with engineered plasmids
• Express fluorescent proteins in yeast cells
• Grow glowing yeast colonies
What You'll Learn
• Genetic engineering in eukaryotic cells
• Yeast transformation techniques
• Gene expression and protein production
Perfect For: Brewers, students, and biotechnology learners.
What’s Included:
Biological Materials
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Saccharomyces cerevisiaeyeast strain
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Preengineered strain of yeast containing GFP plasmid, freeze dried
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Yeast GFP expression plasmid (100ng/uL)
Media & Reagents
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YPD agar
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Selective YPD agar (G418)
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Transformation buffer (0% PEG 8000, 200mM LiAc, 0.1mg/mL salmon sperm DNA)
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YPD broth
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Sterile water
Lab Supplies
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Agar preparation bottle
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Adjustable micropipette (10–100 µL)
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Pipette tips (box of 96)
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5 Sterile inoculation tools
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14 Petri plates
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Tube rack
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Test tube for measuring 50 mL
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Microcentrifuge tubes
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10 Nitrile gloves
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UV filter film
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Blue light
Documentation
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Yeast transformation protocol
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Guide to yeast genetics
Some items in this kit need to be stored in a fridge and a freezer upon you receiving them.
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Reviews
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5 stars
I’d be very appreciative if Odin started selling crispr kits for insects! I love the yeast and bacteria kits I’ve gotten so far, feel free to contact me if insect kits ever happen
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Glowing Brew
Started as a demo for Intro to Biotech course and students made a project out of it to mass produce the protein and add to other food stuffs.


