CRISPR Bacteria Gene Editing Kit
$59.00 - $129.00
Use the same gene-editing tool behind Nobel Prize–winning research to rewrite living DNA - and read the results on an agar plate.
CRISPR-Cas9 changed biology. It's how scientists are developing cures for genetic diseases, engineering drought-resistant crops, and rewriting genomes with a precision that wasn't possible a decade ago. In this kit, you'll use the real thing.
You'll introduce CRISPR components into harmless E. coli, direct Cas9 to cut a specific gene, and repair it with a custom mutation that gives the bacteria antibiotic resistance. The colonies that survive on your selection plates are the ones you successfully edited.
WHAT YOU'LL DO
- Transform bacteria with Cas9, guide RNA, and a DNA repair template
- Target a specific genomic location using guide RNA
- Grow edited and unedited bacteria side by side on antibiotic plates
- Confirm successful edits by comparing colony survival rates
WHAT YOU'LL LEARN
- How CRISPR-Cas9 finds and cuts specific DNA sequences
- How cells repair DNA breaks - and how that repair can be guided
- The role of plasmids in delivering genetic tools into cells
- Why antibiotic selection is a cornerstone of genetic engineering
PERFECT FOR
Students, biohackers, science educators, and anyone who has wondered how CRISPR actually works in practice. No prior lab experience required.
NOTES
• All biological materials are freeze-dried and stable at room temperature for up to one month. Some reagents require refrigeration upon arrival.
• Includes step-by-step experiment guide, CRISPR theory booklet, and plasmid maps.
• The $59 base kit and $129 classroom kit include the same experiment - the classroom version scales supplies for groups of 10+.
WHAT'S INCLUDED:
Biological Materials
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Non-pathogenic E. coli (DH5α strain), Freeze Dried
Cas9 expression plasmid
Guide RNA plasmid
DNA repair template
Media & Reagents
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LB agar media
Selective antibiotic agar media (LB Strep/Kan/Arabinose)
Transformation buffer (25mM CalCl2, 20% PEG 8000)
LB recovery media
Lab Supplies
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14 petri plates
Adjustable micropipette (10–100 µL)
Pipette tips (box of 96)
Microcentrifuge tubes
Tube rack
Inoculation loops
Nitrile gloves
Agar preparation glass bottle
Documentation
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Step-by-step experiment guide
CRISPR theory and protocol booklet
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
Take a look at our Experiment Protocol
Access plasmid maps.
Some items in this kit need to be stored in a fridge and a freezer upon you receiving them.
We Fulfill and Ship in 2-3 days in the US. Overnight is not required and neither are ice packs.
*The REFILL KIT kit does not contain a pipette, pipette tips and a glass bottle for making media. These must be user supplied.
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Reviews
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Good and does what I need it to do
For what I'm doing which is genetically modifying plant DNA for taste and longevity its a good starter kit. I'm not ready to fully go beyond a controlled environment just yet as it'll take me a bit to learn everything its good place to start with. Still learning how it works just to use it safely though such that environmental damage does not occur.
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excellent
I haven't receivend the kit yet, but I'm sure it will meet all my expectations. Thank you!
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Smooth and Straight-forward
AMAZING KIT! I got it super quickly. Inside there are Different elements to play with and explanation guide that teaches you how to work with CRISPR even-tough you have never used it before. I am having so much fun experimenting and exploring genome engineering!
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best
Vraiment top qualité jadore
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Great Intro to Technique
We followed the instructions very carefully and got textbook results on the first go. We took the additional step of trying to grow the naive bug on strep agar (negative results). This kit is a great accompaniment to study of CRISPR. A concise summary of the processes needed to assemble this kit might give students a better feel for its elegance (how the template DNA is produced, for example--a lot of work is needed to put a student in a position to do this kit!). That is not a complaint. This is a really great and enlightening product.
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Tech in the peoples hands
This is an amazing intro to science most would feel out of reach. The quality of materials is better than I had expected. Highly recommended
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CRISPR in the 21st century
The kit contains everything one needs to CRISPR some e. coli. If adequate pre-lab is done, it is quite understandable to most AP Bio students. Although it looks like a bacterial transformation that they have all done previously, they can understand what the Cas9 and the guide RNA is doing. I do have one recommendation - virtually all the kits I buy are for 8 stations/groups - this one is for 7. Drop some of the easily obtained or nice but unnessary things like gloves, 50 ml measuring device, and the microfuge tube rack, and give me enough of the nucleic acids, media, and bacteria to serve 8 stations.
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amazing value
The kit includes everything you need, with an easy to follow protocol for learning CRISPr. The price speaks for itself. 10/10
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Very cool
Directions are a bit vague - yet I still succeed with zero experience! Highly recommend, very cool this is offered to the public.