Genetic Design Starter Kit - Glowing Jellyfish Bacteria
Straightforward and excellent for beginners
Posted by Dory A Muller on 24th Jun 2019
Shipping was awesome and earlier than expected. Instructions were clear and succinct. I was really satisfied with the shelf life of the bacteria and transformation mix--was worried that my delay in completing my experiments would reduce the likelihood of a successful one. Nonetheless, it worked except for the first amp tray...it would be really nice if you could include more educational components that highlights the mechanics and background of each kit item. That way, we can have a wider scope of background knowledge, making the experience that much more enjoyable.
This kit teaches you how to insert a Jellyfish gene, Green Fluorescent Protein(GFP), into bacteria. This allows to bacteria to glow green when you shine light onto them.
Kit comes with everything you need and instructions
Enough for 5 experiments.
LB Amp Agar (Amp 100 µg/ml)
250 mL glass bottle for pouring plates
Disposable transfer pipettes
7- Petri Plates
Inoculation Loops / Plate spreader
6 - 1.5mL microfuge tubes containing LB broth
50mL centrifuge tube for measuring liquid volume
5 - 0.1 mL bacterial transformation buffer (25mM CaCl2, 10% PEG 8000)
UV Filter Sheet and blue light to see the glow
Non-pathogenic E. coli bacteria
5 - 50uL of 25ng/uL GFP plasmid