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6x Dual Color DNA Loading Dye

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  1. Good price, good product

    Posted by Alec Lourenco on 8th Sep 2016

    Works as advertised. I get nice bands when I run with the gel-green like dye also sold by the ODIN.

$5.00
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Product Description

This dye is a pre-mixed loading buffer which contains a combination of two dyes, Dye 1 (pink/red) and Dye 2 (blue). The red dye serves as the tracking dye for both agarose and non-denaturing polyacrylamide gel electrophoresis. The two dyes separate upon gel electrophoresis; the red band is the major indicator and migrates similarly to Bromophenol Blue on agarose gels. Specifically chosen, this dye does not leave a shadow under UV light. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. The dye also contains Ficoll, which creates brighter and tighter bands when compared to glycerol loading dyes.

Storage Temperature

25°C

1X Buffer Components

2.5% Ficoll®-400
10mM EDTA
3.3mM Tris-HCl
0.08% SDS
0.02% Dye 1
0.001% Dye 2
pH 8.0@25°C

 

Quality Assurance Statement

  • This dye has been assayed for Non-Specific DNase Activity (16 hour), Exonuclease Activity (Radioactivity Release), Endonuclease Activity (Nicking) and RNase Activity (Extended Digestion).