Change the color of plant leaves using modern genetic engineering techniques.
In this experiment you will introduce new DNA into plant cells to modify pigment production. By altering how plants produce anthocyanins and chlorophyll-related pathways, leaf coloration changes become visible as the plant grows.
This kit demonstrates the fundamentals of plant genetic engineering and DNA delivery.
NOTE: We cannot ship live plants internationally. Please do not check the box for a live plant if you are not in the United States.
• Introduce engineered DNA into plant tissue
• Trigger pigment pathway changes
• Observe altered leaf coloration during growth
• How plant genetic engineering works
• DNA delivery methods for plant cells
• Pigment biosynthesis pathways in plants
• How traits can be modified through genetics
Biological Materials
Plant transformation Agrobacterium w/ RUBY Plasmid, Freeze Dried
Nicotiana tabacum plant or seeds (depending on kit version)
Media & Reagents
Injection media concentrate
LB Rifampicin/ Spectinomycin agar
Sterile water
5 Sprouting disks
Bag of soil
Lab Supplies
7 Petri plates (2 pack for classroom kit)
2 Test tubes 50mL
1 Test tube for measuring 50mL
5 Test tubes 15mL
5 Blunt syringes (15 for classroom kit)
5 Pasteur pipettes
1 Pot
1 Plastic tub for sprouting plants
Agar preparation glass bottle
10 Sterile inoculation loops
10 Nitrile gloves (3 pack for classroom kit)
Documentation
Step-by-step plant transformation protocol
Guide to plant pigment genetics
A. Tumefaciens highly viable for plant transformation protocols, and worked OK for my plant calli. In the middle of transgenically modifying calli for my 7th grade sci fair!
Incredibly simple to use and explain to school children and college students toward the necessary understanding of gene ? control and phenotypical manifestation.
I just recently bought this to keep my mind occupied and man oh man this kit rocks my world. I have now developed a passion and love for genetic engineering. I highly recommend you buy this!!!