
E. coli DH5a strain freeze dried
Strain for DNA replication when cloning plasmids in bacteria.
For creating competent cells see here: http://openwetware.org/wiki/Preparing_chemically_competent_cells
F– endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK–mK+), λ–
- A Hoffman-Berling 1100 strain derivative (Meselson68)
- Promega also lists phoA
- Thiamine, arginine auxotroph
- Lactose nonutilizing
- Nalidixic acid resistant
- Recombination-deficient
- Relaxed phenotype
- Endonuclease A deficient
- Constitutive deoxyribose synthesis, good for cloning large plasmids.
- Amber suppressor UAG>CAG(Gln).
- Slow growth in M9 medium due to purB-20
- References:
- FOCUS (1986) 8:2, 9.
- Hanahan, D. (1985) in DNA Cloning: A Practical Approach (Glover, D.M., ed.), Vol. 1, p. 109, IRL Press, McLean, Virginia.
- Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87: 4645-4649 PMID 2162051.
- Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.
**Perishables included in this kit have been Freeze Dried. They will be viable over a month at room temperature**
Reviews
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Reliable DH5a E. coli!
Fast delivery, great customer service, and chock full of quality viable cells! Way better than rolling the dice with eBay!
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Excellent
No problem bringing them back to life and inserting plasmids for my experiments.
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easy ordering process
Fast easy ordering. They always answer any questions I have. I’ll be ordering from here again.
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Life Finds a Way
I had some DH5a carrying a plasmid, but after several iterations of stressing and subculturing, I was unable to drive it out. For the price, this option was a much better use of my time. Despite the product image, I wasn't expecting it to come with a backup; a pleasant surprise! Used this along with some odin ampicillin for transformations and confirmed with controls that they both work.