A comprehensive biotechnology learning program combining foundational bioengineering and advanced human tissue engineering.

Students begin with basic genetic engineering experiments and progress into mammalian cell culture and tissue engineering techniques used in modern biomedical research.

What You'll Do

• Perform foundational bioengineering experiments
• Culture and manipulate human cells
• Learn tissue engineering concepts

What You'll Learn

• Genetic engineering fundamentals
• Mammalian cell biology
• Tissue engineering methods

Perfect For: Students pursuing advanced biotechnology training.

What’s Included

Biological Materials

• HEK 293 Human Embryonic Kidney Cell Line

• DNA constructs for gene expression (lacZ gene)

• Engineered bacterial strains (pDusk/pDawn Biosensors Kit), Non-pathogenic, freeze dried

• Engineered bacterial strains (E. coli DH10B strain w/ pJE202 plasmid), Non-pathogenic, freeze dried

• Non-pathogenic E. coli (DH5α strain), freeze dried

• Cas9 expression plasmid

• Guide RNA plasmid

• DNA repair template

• Fluorescent protein plasmid (GFP)

Media & Reagents

• Mammalian cell culture media (100mL of L15 + 10% Fetal Bovine Serum + Amp/Strep/Gent)

• Transfection reagents

• 50mL Trypsin EDTA Solution

• 100uL G418 200mg/mL

• LB agar media

• LB agar media with selective antibiotics

• Transformation reagents

• Colored water for pipetting practice

Lab Supplies

• 4 25cm Cell Culture Treated Flasks

• 10 Syringes and Filters

• 4 12 Well culture plates

• Sterile inoculation tools

• Nitrile gloves

• Microcentrifuge tubes

• Tube rack

• Sterile inoculation loops

• Nitrile gloves

• Orange film and Blue light

• Tube for measuring 50 mL

• Scale and weigh boats

• Adjustable micropipette (0.5–10 µL) (IF SELECTED)

• Adjustable micropipette (10–100 µL) (IF SELECTED)

• Adjustable micropipette (100–1000 µL) (IF SELECTED)

• Pipette tips (0.5–10 µL) (box of 96) (IF SELECTED)

• Pipette tips (10–100 µL) (box of 96) (IF SELECTED)

• Pipette tips (100–1000 µL) (box of 96) (IF SELECTED)

• Temperature controlled heat mat (IF SELECTED)

• Inverted Microscope with 3MP USB camera (IF SELECTED)

Digital Materials

• Bioengineering 101 lecture series

• Human Tissue Engineering 201 lectures

• Experiment protocols and course materials

Bioengineering 101

This kit and class teaches you everything you need to know to do Bioengineering. No experience needed. Despite the class not being live you can participate in an Online Classroom where you can interact with instructors when you need them to help answer any questions you may have.

The class has 8 lectures and experiments. The videos can be viewed remote online at anytime and you can follow along with hands-on experiments that we ship to you. The value of the supplies alone is worth the cost of the class! We will send all you all the class links and info once you register.

The class is remote so you can do it from anywhere. 

This class will take a few hours a week to perform experiments that can be done at your own pace.

A link to the last classes syllabus can be found here

A link to Molecular Biology of the Cell can be found here

Example Experiment

Example Lecture

Class includes guest lectures from

Dr. George Church

Dr. Kate Adamala

Materials Included

0.5-10 uL pipette*
10-100 uL pipette*
100-1000 uL pipette*
0.5-10 uL tips*
10-100 uL tips*
100-1000 uL tips*
Microcentrifuge tube rack
Petri dishes
Scale and weigh boats
Glass bottle
Nitrile Gloves
Orange film and Blue light
Microcentrifuge tubes
Inoculation Loops
LB agar
50 mL tube for measuring
Colored water for pipetting practice

Bioluminscent Bacteria Grow Kit

GFP Bacteria Kit

CRISPR Kit

pDusk/ pDawn Biosensors Kit

*Items with an asterisk must be selected to receive with your kit.

You will learn

How to Pipette
How to Pour plates
Sterile technique 
Hand Culture
How to Use a Scale

How to Streak plates and Culture bacteria

Growing Bioluminescent Bacteria

Bacterial Transformation
Yeast Transformation
Biosensors
Postive/Negative Controls

All About CRISPR

Bioengineering 201

This class is pre-recorded but a teacher will be available to answer your questions.

In this class you will learn how to culture, grow and genetically modify HEK 293 human cells in culture. No experience needed but some experience is preferred. We will not be teaching basic skills like pipetting which are needed for this class.

PLEASE NOTE: Can do international shipping but because of time requirements the cost is generally around $300-$400. Shipping to Canada is only around $100 extra. Email us to get quotes.

This class is 4 weeks long and will cover

Week 1

Culturing Human Cells

Week 2 

Maintenance and Splitting Passaging of Human Cells

Week 3

Antibiotic Screening

Week 4 

Genetic Engineering

 
WARNING You cannot do this class without pipettes and an inverted microscope! 

*Items with an asterisk must be selected to receive with your kit.

#Not provided is > 90% Isopropyl Alcohol for cleaning

Microscope is monocular inverted microscope with 4x, 10x and 20x objectives and LED illumination. 3MP digital camera interfaces with AMScope Software.

**IN ORDER TO PROVIDE THE BEST QUALITY HUMAN CELLS, SHIPPING TIME MAY VARY**