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Grow Bioluminescent E. coli Kit

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Product Description

This kit includes a DH10B based strain of E. coli that contains the Lux operon in the pJE202 plasmid. It also contains a 250mL glass bottle and enough media to grow up 8 cultures.(items pictured not included)

 

How it works:

Add one tube of the media to the glass bottle.

Fill bottle with distilled water and shake until the media is dissolved.

Add small amount of bacteria to the bottle and let grow overnight at room temperature. Make sure the bottle never heats up above ~30C(86F) as the bacteria will not luminess.

The process of bioluminescence needs oxygen so give a gentle swirl to the bottle in a dark room and watch the bacteria glow!  The darker the room the better.

 The protocol for the kit can be found here: http://www.the-odin.com/bio-luminescent-bacteria/