Grow Bioluminescent E. coli Kit
Super easy and awesome experiment
Posted by Unknown on 9th May 2019
Kit contains a good quantity of all the materials necessary to grow several batches of bioluminescent microbes. Within 24 hours of my first batch, I had results similar to the pictures! Crazy cool and very easy. Could be a very fun science experiment for youngsters.
This kit includes a DH10B based strain of E. coli that contains the Lux operon in the pJE202 plasmid. It also contains a 250mL glass bottle and enough media to grow up 8 cultures.(items pictured not included)
How it works:
Add one tube of the media to the glass bottle.
Fill bottle with distilled water and shake until the media is dissolved.
Add small amount of bacteria to the bottle and let grow overnight at room temperature. Make sure the bottle never heats up above ~30C(86F) as the bacteria will not luminess.
The process of bioluminescence needs oxygen so give a gentle swirl to the bottle in a dark room and watch the bacteria glow! The darker the room the better.
The protocol for the kit can be found here: http://www.the-odin.com/bio-luminescent-bacteria/