Learn the fundamentals of CRISPR gene editing with a simplified experiment.
This streamlined version of the CRISPR kit focuses on the core principles of genome editing while reducing experimental complexity, making it ideal for beginners and classrooms.
• Introduce CRISPR tools into bacteria
• Perform a simplified gene editing experiment
• Identify edited bacteria on selective plates
• The basic mechanism of CRISPR-Cas9
• DNA targeting using guide RNA
• Selection of genetically edited cells
Biological Materials
Non-pathogenic E. coli (DH5α strain) bacteria, freeze dried
Simplified CRISPR plasmid system (Cas9 expression plasmid, Guide RNA plasmid, DNA repair template)
Media & Reagents
LB agar
Selective LB agar (Strep/Kan/Arabinose)
Transformation buffer
Sterile water
Lab Supplies
7 Petri plates
Agar preparation bottle
5 Microcentrifuge tubes
9 Transfer pipettes
5 Sterile inoculating loops
10 Nitrile gloves
Documentation
Beginner CRISPR protocol
Introduction to gene editing
**Perishables included in this kit have been Freeze Dried. They will be viable up to a month at Room temperature**
I bought this as part of my own way to start learning about CRISPR and it blew my mind how well written and easy to follow the manual and information is, now I really want to learn more from their other kits and classes