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E. coli DH5a

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Product Description

E. coli DH5a strain.

Strain for DNA replication when cloning plasmids in bacteria.

For creating competent cells see here: http://openwetware.org/wiki/Preparing_chemically_competent_cells

Genotype

F endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rKmK+), λ

  • A Hoffman-Berling 1100 strain derivative (Meselson68)
  • Promega also lists phoA
  • Thiamine, arginine auxotroph
  • Lactose nonutilizing
  • Nalidixic acid resistant
  • Recombination-deficient
  • Relaxed phenotype
  • Endonuclease A deficient
  • Constitutive deoxyribose synthesis, good for cloning large plasmids.
  • Amber suppressor UAG>CAG(Gln).
  • Slow growth in M9 medium due to purB-20
  • References:
    • FOCUS (1986) 8:2, 9.
    • Hanahan, D. (1985) in DNA Cloning: A Practical Approach (Glover, D.M., ed.), Vol. 1, p. 109, IRL Press, McLean, Virginia.
    • Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87: 4645-4649 PMID 2162051.
    • Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.