- Place the strain in LB for bacteria and Knockout media for yeast or other appropriate media
- Add the appropriate antibiotic for bacteria in the correct concentration, this can be found by searching the internet for “<antibiotic name> working concentration” where <antibiotic name> is the name of the antibiotic need for plasmid selection
- If bacteria or yeast don’t grow or take a long time to grow, first create a starter culture by inoculating media without an antibiotic for 1-2 hours and allowing the strain to grow then adding 100uL / mL to the selection media.
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