Live Class - Human Tissue Culture and Engineering
This is a Live class that will take place over 4 weeks dates are on Sundays -Jan 9th, Jan 16th, Jan 23 & Jan 30at 11AM US Central time. Classes will also be recorded for later use.
Your teacher will be Josiah Zayner Ph.D.
In this class you will learn how to culture, grow and genetically modify HEK 293 human cells in culture. This is a new version of our human cells kit that puts the lacZ gene into the human cells so that they create a blue color change. No experience needed but some experience is preferred. We will not be teaching basic skills like pipetting which are needed for this class.
You will receive a code that allows you to login to the classroom with your kit.
Can do international shipping but because of time requirements the cost is generally around $300-$400. Shipping to Canada is only around $100 extra. Please contact us if you wish to have the kit shipped outside the US.
This class is 4 weeks long and will cover
Culturing Human Cells
Maintenance and Splitting Passaging of Human Cells
Items Included (WARNING You cannot do this class without pipettes and an inverted microscope!!!)
1 - 12.5cm Cell Culture Treated Flasks with HEK293 filled with L15 + 10% Fetal Bovine Serum + Amp/Strep/Gent
- 4 - 12.5cm Cell Culture Treated Flasks
100mL of L15 + 10% Fetal Bovine Serum + Amp/Strep/Gent (Media for growing cells)
10 - Syringes and Filters
4 - 12 well plates
20 pairs nitrile gloves
50mL Trypsin EDTA Solution
100uL G418 200mg/mL
50 Mircocentrifuge tubes
10 - 50mL tubes
10 - 10mL tubes
4 sided tube rack
#Not provided is > 90% Isopropyl Alcohol for cleaning
*Microscope is monocular inverted microscope with 4x, 10x and 20x objectives and LED illumination. 3MP digital camera interfaces with AMScope Software.
***If you order in time for Christmas the kit will be shipped before then but we won't ship the cells to arrive until near the start of the class as they can die easily***