E. coli strain BL21(DE3)
This strain is used for protein (over)expression from a T7 promoter.
For creating competent cells see here: http://openwetware.org/wiki/Preparing_chemically_competent_cells
Genotype can be found here: http://openwetware.org/wiki/E._coli_genotypes#BL21.28DE3.29
**Perishables included in this kit have been Freeze Dried. They will be viable over a month at Room temperature**
Purchased for my undergrad college research. Put in the order at noon EST July5th and was delivered to Cleveland Ohio July 11th at 10am EST. Two screwtop-microvials of freeze-dried BL21, multiple plastic inocculating loops, a screwtop-microvial of sterile water, plastic droppers, and stickers came in the mail. Incubated with both falcon tubes and on Petri dishes with a 1.75% agar/lb broth mixture. Exceptional growth after 16 hours at 36-38C in an incubator (igloo cooler with reptile heating pad). Absolutely will purchase again... in fact I am putting in a new order now.
The T7 overexpression has helped a lot with a few of my projects. Great for creating lots of a single product.