E. coli BL21 (DE3)

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E. coli strain BL21(DE3)

This strain is used for protein (over)expression from a T7 promoter.

For creating competent cells see here:

Genotype can be found here:


**Perishables included in this kit have been Freeze Dried. They will be viable over a month at Room temperature**


17.78 (cm)
17.78 (cm)
17.78 (cm)


  • 5

    Posted by Brady J on 17th Jul 2022

    Purchased for my undergrad college research. Put in the order at noon EST July5th and was delivered to Cleveland Ohio July 11th at 10am EST. Two screwtop-microvials of freeze-dried BL21, multiple plastic inocculating loops, a screwtop-microvial of sterile water, plastic droppers, and stickers came in the mail. Incubated with both falcon tubes and on Petri dishes with a 1.75% agar/lb broth mixture. Exceptional growth after 16 hours at 36-38C in an incubator (igloo cooler with reptile heating pad). Absolutely will purchase again... in fact I am putting in a new order now.

  • 5
    Favorite Strain

    Posted by Jake on 17th Aug 2016

    The T7 overexpression has helped a lot with a few of my projects. Great for creating lots of a single product.