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Taq Polymerase Master Mix 2x - 1mL - 40 reactions

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Product Reviews

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  1. Even better than the old stuff (which was great!)

    Posted by Jason on 28th Nov 2018

    I was super satisfied with the old 5x Taq mix, so I was nervous to try a new one, but this stuff is AMAZING. Super bright bands, easy to work with, not as bubbly as what I was using before which is nice. I usually run 30uL reactions so it's not super relevant to me but just so you know, the reaction setup over there on the right is assuming 5x master mix, not 2x. At least as of this writing, they're probably fixing it right now and I'll look like a fool for years to come :D

  2. FANTASTIC!!!

    Posted by Unknown on 11th May 2017

    I work in a formal molecular biology lab and decided to give this product a go. When I ordered it, I was super hesitant about its shipping method (not frozen). I received it yesterday and tested today in the lab it along with its accompanying ladder (100bp). Both products had a better resolution in the gel than commercial ($100+ each) products. We compared the ladder to 1kBReady GeneRuler as well as Thermo 50bp ladder. This one was cleaner and stronger looking. The master mix was tried on a very difficult template (we had spent weeks trying to optimize it--which we finally did) and this master mix gave us a nicer looking band. Everyone in my lab now wants to steal it from me. Haha.
    Keep up the good work guys

  3. Great price, great product

    Posted by Alec Lourenco on 8th Sep 2016

    Yields a very pure product. Very satisfied.

  4. awesome price, the backbone of my PCR

    Posted by David on 12th Aug 2016

    Got here quick works perfectly and amazing price.

$20.00
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Product Description

You receive 1mL of 2x Taq Master Mix. All you need is the DNA Template and Primers, this mix contains everything else.

You can find primers for amplifying your own DNA with our DNA purification kit. This will allow you to learn about forensic identification of human beings.

Good for

40 - 50uL PCR Reactions

80 - 25uL PCR Reactions

To use this polymerase to amplify your DNA requires a PCR Thermocycler.

For PCR

Component 25uL Reaction 50uL Reaction Final Concentration
10uM Forward Primer 0.5uL 1uL 0.2uM
10uM Reverse Primer 0.5uL 1uL 0.2uM
5x Taq Master Mix 5uL 10uL 1x
DNA Template Depends Depends Your total conc. should be <500ng
Water to 25uL to 50uL  

 

 

 

 

 

 

PCR Settings

Step Temperature Time
Initial Denaturation 95C 30s
25-30 cycles of the following

95C

55C

68C

15s

30s

1 minute/kilobase

Final Extension 68C 5 minutes
Hold at 4C if needs be