Taq Polymerase Master Mix 2x - 1mL - 40 reactions
Even better than the old stuff (which was great!)
Posted by Jason on 28th Nov 2018
I was super satisfied with the old 5x Taq mix, so I was nervous to try a new one, but this stuff is AMAZING. Super bright bands, easy to work with, not as bubbly as what I was using before which is nice. I usually run 30uL reactions so it's not super relevant to me but just so you know, the reaction setup over there on the right is assuming 5x master mix, not 2x. At least as of this writing, they're probably fixing it right now and I'll look like a fool for years to come :D
Posted by Unknown on 11th May 2017
I work in a formal molecular biology lab and decided to give this product a go. When I ordered it, I was super hesitant about its shipping method (not frozen). I received it yesterday and tested today in the lab it along with its accompanying ladder (100bp). Both products had a better resolution in the gel than commercial ($100+ each) products. We compared the ladder to 1kBReady GeneRuler as well as Thermo 50bp ladder. This one was cleaner and stronger looking. The master mix was tried on a very difficult template (we had spent weeks trying to optimize it--which we finally did) and this master mix gave us a nicer looking band. Everyone in my lab now wants to steal it from me. Haha.
Keep up the good work guys
Great price, great product
Posted by Alec Lourenco on 8th Sep 2016
Yields a very pure product. Very satisfied.
awesome price, the backbone of my PCR
Posted by David on 12th Aug 2016
Got here quick works perfectly and amazing price.
You receive 1mL of 2x Taq Master Mix. All you need is the DNA Template and Primers, this mix contains everything else.
You can find primers for amplifying your own DNA with our DNA purification kit. This will allow you to learn about forensic identification of human beings.
40 - 50uL PCR Reactions
80 - 25uL PCR Reactions
To use this polymerase to amplify your DNA requires a PCR Thermocycler.
|Component||25uL Reaction||50uL Reaction||Final Concentration|
|10uM Forward Primer||0.5uL||1uL||0.2uM|
|10uM Reverse Primer||0.5uL||1uL||0.2uM|
|5x Taq Master Mix||5uL||10uL||1x|
|DNA Template||Depends||Depends||Your total conc. should be <500ng|
|Water||to 25uL||to 50uL|
|25-30 cycles of the following||
|Final Extension||68C||5 minutes|
|Hold at 4C if needs be|