Gel Green like DNA Stain for Gels 10,000x
As good as ethidium bromide!
Posted by Skinzo on 7th Dec 2018
I first received a small free sample of this and was so impressed by the quality of the gel images that I went back online and bought more. While I have always preferred to use ethidium bromide and UV transillumination, I do a lot of experiments with young scientists and need safer alternatives. This dye, along with the yellow glasses and blue LED, produces clear images of DNA bands resolved by agarose gel electrophoresis..and I don't worry about subjecting my young proteges to harmful UV, nor do I have to worry about ethidium bromide disposal!
patience is a virtue
Posted by JK on 20th Nov 2018
I ordered the stain to be shipped across the Pacific. I was anxious that quality might deteriorate. I ran electrophoresis for 3 times, unable to observe any bands. I eventually discovered that you need to see them through a yellow goggle. On my 4th attempt, I had my goggle ready, but still nothing for the first 10 minutes or so. I was in despair, but nonetheless tried for one last time before packing my stuffs. Alas, when I lit my gel not from bottom, but from slant up, I could see my bands!
Did the stain take time to bind with DNA?
Posted by Alec Lourenco on 8th Sep 2016
Very cheap alternative to other DNA stains like Sybr Safe. I add 5 ul and use UV light to visualize my gels. When stored at -20 degrees C, the stain turns a brownish color after freezing, but this does not seem to have any effect on its viability.
I lost my gel green, Odin saved the day.
Posted by David on 12th Aug 2016
I lost my gel green a couple of weeks ago, and had to order more in a hurry. I had some time sensitive experiments running and without gel green I'm blind. The Odin rushed more to me in time to save the research.
This DNA stain is a safe class of fluorescent dyes, used for agarose or polyacrylamide gel staining to visualize DNA or RNA. You can use it the same way as you would use ethidium bromide. It emits green fluorescence when bound to DNA and red fluorescence when bound to RNA when exposed to UV light or green fluorescence when exposed to blue light. It has two excitation maxima: 290nm and 490nm. Most blue LEDs work great.
100uL per unit.
- Prepare a 50mL agarose gel
- Add 5 μL Gel Stain to the agarose while it is hot
If you have trouble seeing bands on your illumination setup try using 10uL of the DNA stain.