Engineer Your Own Green Fluorescent Yeast Kit
Genetic engineering involves modifying an organism’s DNA to deliberately change an aspect of the organism for a particular purpose. This kit demonstrates the power and simplicity of genetic engineering by adding plasmid DNA to the yeast Saccharomyces cerevisiae so that it turns a fluorescent green color from expression of the Green Fluorescent Protein (GFP).
The yeast in this kit are laboratory yeast that don't ferment well. This kit and DNA only work with the laboratory yeast provided.
This kit requires ~10 hours of work over the course of at least 2 days. It includes everything you need and teaches many molecular biology and gene engineering techniques.
- CM Agar Minus Uracil
- YPD Agar
- S. ceravisiae strain
- Glass Bottle
- Inoculation Loops
- 10-100uL Pipette
- Pipette Tips
- 14 Plates
- Microcentrifuge tube rack
- 5 Pairs Nitrile Gloves
- 25 microcentrifuge tubes
- 50 mL Tube for Measuring Liquid Volume
- TransEyeLuminator Glasses with blue lights
- Yeast transformation buffer 100mM LiAc 30% PEG 8000 10% DMSO
- GFP plasmid
- BY4741 Yeast Strain