DIY Bacterial CRISPR Refill/Classroom Kit
Great for the Classroom
Posted by George on 11th Jul 2017
This kit was a success, directions were easy to understand and would definitely purchase again!
Hard to understand
Posted by Gage on 4th Feb 2017
This was a really go experiment to do. It was hard to understand the instructions though and the only way I knew what to do was to talk to my professors. On top of all that we were all afraid that the E. coli was pathogenic and was going to kill us all. We did not see that it was non pathogenic until after the experiment. Other than all that though it was a thoroughly engaging, interesting, and successful experiment. It helped me to figure out that I want to get a microbiology degree.
This kit provides the media, DNA, bacteria and plasticware to perform a bacterial CRISPR experiment. 1 item is enough for 5 experiments.
- 50uL of Cas9 plasmid
- 50uL of gRNA plasmid
- 50uL template DNA
- Strep/Kan/Arabinose Agar Media
- LB Agar Media
- LB Media
- 20 microcentrifuge tubes
- 14 plates
- Non-pathogenic E. coli bacteria
- Inoculation loops
- 750uL Bacterial Transformation Buffer
*This kit does not contain pipette, pipette tips and glass bottle for making media. These must be user supplied.
Some items in this kit need to be stored in a fridge and a freezer upon you receiving them.